In this study, we determine cross-reactivity of a variety of steroid and steroidal compounds (including anabolic steroids) to the Roche Diagnostics Elecsys immunoassays for cortisol, DHEA sulfate, estradiol, progesterone, and testosterone, and compare these results to other cross-reactivity studies.

2019年4月11日 · 是一种利用紫外交联技术，将RNA与蛋白之间互作的弱作用力（如范德华力、氢键）转化为强作用力（共价键），并连用免疫沉淀，探究RNA与蛋白互作位点的分子生物学技术。 基于CLIP技术可以在整个转录组水平上检测RNA结合蛋白的结合位点或RNA修饰（m6A）位点。 CLIP的基本实验流程： 1紫外交联细胞，使RNA与蛋白之间形成共价结合。 2用特异性抗体调取特定蛋白与RNA复合物。 3用RNase消化掉没有结合蛋白的RNA，并用RNA连接酶连接3’端的接 …

Exposure to drugs of abuse is frequently assessed using urine drug screening (UDS) immunoassays. Although fast and relatively inexpensive, UDS assays often cross-react with unrelated compounds, which can lead to false-positive results and impair patient care.

2014年7月14日 · One limitation of steroid hormone immunoassays is interference caused by compounds with structural similarity to the target steroid of the assay. Interfering molecules include structurally related endogenous compounds and their metabolites as well as drugs such as anabolic steroids and synthetic glucocorticoids.

交联试剂 通过在两个或多个生物分子上的特定氨基酸官能团之间因其相邻端相互作用形成化学键，从而将相互作用蛋白，结构域或肽共价连接到一起。 市售交联试剂具有多种特性，包括： 官能团特异性 —交联剂分子携带靶向胺，巯基，羧基，羰基或羟基的反应性部分。 同型双功能或异型双功能 —分子的两端具有相同的反应部分 [称为同型双功能分子；如下所示的上层分子 (A/A) ,例如 DSS]，可交联相同的残基，而异型双功能交联剂上每个反应基团靶向不同蛋白上的不同官能 …

2022年3月17日 · Cross-reactivity in ELISA can occur through non-specific binding, or substances that adsorb to the solid phase and cause high background noise or false signals. It can also occur when...

2014年2月1日 · Cross-reactivity (CR) in multiplexed immunoassays has been unexpectedly difficult to mitigate, preventing scaling up of multiplexing, limiting assay performance, and resulting in inaccurate and even false results, and wrong conclusions. Here, we review CR and its consequences in single and dual antibody single-plex and multiplex assays.

2021年4月1日 · Implied in its name, the TCR assay is intended to identify both expected and unexpected sites of cross reactivity of mAb/mAb-like molecules. Several platforms, using a variety of approaches, have been developed in recent years to provide a comprehensive screen for binding across a substantial portion of the human and/or animal proteome.

Cross-reactivity is the ability of the antibody to bind other structures than the target analyte. These structures have often a high similarity or homology to the analyte. The term cross-reactivity is used if the cross-reacting substance is known and the ability of cross-reacting has been proved e.g. by testing the competitive concentration of ...

2023年12月12日 · All assays to be multiplexed in an array must be tested for various types of cross-reactivity to determine if the reagents are specific enough to give a true positive signal. For example, the following types of cross-reactivity can occur: